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. Author manuscript; available in PMC: 2010 Jun 3.
Published in final edited form as: Nature. 2009 Nov 18;462(7273):660–664. doi: 10.1038/nature08599

Figure 4. Effect of PP2C mutations on ABA response phenotypes and kinase activities of SnRK2s.

Figure 4

a, In-gel kinase assay showing the activities of SnRK2s in the abi1/hab1/abi2 (i2) and abi1/hab1/pp2ca (ca) triple mutants. The snrk2.2/2.3/2.6 was used as a control. GST-fused ABF2 fragment (amino acids Gly73 to Gln119) was used as the phosphorylation substrate. The expected positions of SnRK2.6 and SnRK2.2/2.3 are indicated by arrow and arrowhead, respectively. Radioactivities of the upper and lower bands were normalized, taking the radioactivity of the upper band in WT as 1 (mean ± s.e.m., n = 3). b, The PP2C triple mutants show ABA hypersensitivity during germination and early seedling development. Photograph of plants of the indicated genotypes growing on MS medium with 3% sucrose for 14 d. c, The percentage of seedlings with green cotyledons 6 d after the end of stratification is shown (mean ± s.e.m., n = 3).