Figure 2. Glucose Metabolism in the Mice with Acute Liver-Specific Ablation of ARNT.

(A) The ARNT flox/flox mice were injected via the tail vein with adenovirus Ad-Cre vector to knock out ARNT in liver (L-ARNT KO). Age- and sex-matched ARNT flox/flox mice were injected with Ad-CMV as controls (Control). Six days after injection, hepatic mRNA levels of ARNT were measured by real-time RT-PCR.

(B) ARNT protein levels were measured from a 30 mg aliquot of total homogenates from liver by western blot analysis. Each lane represents the liver sample from an individual mouse.

(C and D) Fed blood glucose and insulin levels were measured in Control and L-ARNT KO mice from the FNC, FHF, and MNC groups.

(E) Glucose tolerance tests (GTTs) were performed in Control and L-ARNT KO mice from the FNC, FHF, and MNC groups.

(F) Pyruvate challenge tests in Control and L-ARNT KO mice from the FHF and MNC groups were used to assess hepatic gluconeogenesis.

(G) The area under the curve (AUC) for serum glucose during the pyruvate challenge tests from FHF and MNC groups were calculated using the linear trapezoidal rule, subtracting the baseline value from each time point.

(H) Glucose infusion rates for Control and L-ARNT KO mice from the FNC group during the euglycemic clamp were measured.

(I) Basal and insulin-suppressed hepatic glucose production in Control and L-ARNT KO mice from the FNC group were measured during the euglycemic clamp. The black line with black boxes in the figure represents the data obtained from L-ARNT KO mice. The dotted line with open boxes in the figure represents the data obtained from Control mice. Data are means ± SEM of values from five or six mice. Asterisks indicate p < 0.05. Data from Figures 2C–2I are means ± SEM of values from five or six mice. Asterisks indicate p < 0.05.