Figure 3. Effect of L-ARNT KO on the Expression of Hepatic Gluconeogenic Enzymes.
(A) mRNA levels of gluconeogenic genes from the livers of the three experimental groups were measured by real-time RT-PCR. The livers of mice from each group were obtained in the fed stage at day 6 after injection. Data are representative of results obtained from five or six mice in each group.
(B) mRNA levels of transcription factors and coactivators involved in the regulation of PEPCK, G6Pase, and FBP1 were measured by real-time PCR. Data are representative of results obtained from six mice in the FNC group.
(C) C/EBPα protein level was measured by immunoblotting in the livers of L-ARNT KO mice from the FNC group. Each lane represents the liver protein extract from an individual mouse. Quantification data of C/EBPα protein were obtained from five mice. Asterisks indicate p < 0.05.