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. Author manuscript; available in PMC: 2010 Nov 6.
Published in final edited form as: Circ Res. 2009 Sep 24;105(10):956–964. doi: 10.1161/CIRCRESAHA.109.198515

Figure 4. PDE1 mRNA and protein expression in human, rat, and mouse ventricular tissues and isolated cardiomyocytes.

Figure 4

(A–C) RT-PCR results showing PDE1A, PDE1B, and PDE1C mRNA expression in adult human, rat, and mouse heart tissue compared to indicated-controls (mouse brain for PDE1A and 1B or mouse testis for PDE1C). RT-PCR data was quantified by densitometry in a linear range from three independent samples, which were normalized to GAPDH mRNA levels and expressed relative to human hearts (AU=arbitrary units). (D) Representative western blot showing relative PDE1A, PDE1B, and PDE1C protein levels in human, rat, and mouse hearts, compared to respective controls (Brain for PDE1A and PDE1B; Testis for PDE1C). GAPDH was used to normalize protein loading. (E and F) PDE1 expression in isolated neonatal and adult cardiomyocytes (NRVM and ARVM). RT-PCR showing relative PDE1A, 1B, and 1C mRNA levels in NRVM, ARVM, and rat hearts, compared to respective controls (E). Western blot depicting relative PDE1A, 1B, and 1C protein levels in NRVM, ARVM, compared to rat hearts and respective controls. GAPDH was used to normalize mRNA and protein expression.