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. Author manuscript; available in PMC: 2010 Jan 7.
Published in final edited form as: Nature. 2009 Jun 3;459(7249):996–999. doi: 10.1038/nature08119

Figure 3.

Figure 3

Duox activity is required for wound margin H2O2 production and leukocyte recruitment. (a) Wound margin H2O2 after morpholino mediated duox knockdown (MO1-duox) or injection of a corresponding 5-misprime morpholino (5-MP) imaged 17 min pw. Inset: RT-PCR of a duox mRNA region flanking the targeted splice site. (b) Quantification of wound margin [H2O2]. (c) Injured tail fins of mpo::GFP9 larvae injected with MO1-duox, or 5-MP (42 min pw). Coloured lines: leukocyte tracks. (d) Quantification of leukocyte recruitment. Error bars: SEM of indicated number of larvae (brackets). ** P < 0.01, *** P < 0.001 (vs. control). Scale bars: 100 μm.