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. Author manuscript; available in PMC: 2010 Jan 7.
Published in final edited form as: Immunity. 2009 Mar 5;30(3):384–396. doi: 10.1016/j.immuni.2008.12.020

Figure 4. High-Affinity LFA-1 Dots Are Devoid of F-actin and pTyr and Support T Cell Crawling Independent of Src Signaling.

Figure 4

(A) T cells crawling on activated HUVEC were fixed and costained for HA-LFA-1 (green) and F-actin (red, top) or pTyr (red, bottom). Images are representative of 30 crawling T cells. Scale bars represent 6 μm.

(B) Migratory phenotypes of T cells pretreated with PP2 (10 μm) or a carrier interacting with activated HUVEC.

(C) Effects of PP2 pretreatment on T cell adhesion and crawling on ICAM-1+CXCL12 analyzed as in Figure 3B. Right: effects of PP2 on TCR-induced (anti-CD3-stimulated) lymphocyte spreading on ICAM-1.

(D) HA-LFA-1 dot density in PP2 and control treated T cells adhered and crawling on ICAM-1+CXCL12. Values are the mean ± SD of 15 cells in each group.

(E) Migratory phenotypes of T cells pretreated with the DAG-PKC inhibitor, GF-109203x (5 μm) or a carrier interacting with activated HUVEC overlaid with CXCL12.

Values in experiments shown in (B), (C), and (E) represent the mean ± range of two fields.