Figure 3.

EGCG increases Akt (Ser⁴⁷³) and FoxO1 (Thr²⁴) phosphorylation in a dose-dependent manner. BAECs were serum-starved overnight and then treated for 10 min with the indicated doses of EGCG. Cell lysates were then analyzed my immunobloting with phospho- and nonphospho-specific antibodies. A, Representative immunoblot of Akt phosphorylation stimulated with the indicated doses of EGCG. B, Quantification (mean ± sem) of multiple independent experiments shown in panel A (*, P < 0.01 by ANOVA and Dunnett’s posttest). C, Representative immunoblot of FoxO1 phosphorylation stimulated with the indicated doses of EGCG. D, Quantification (mean ± sem) of multiple independent experiments shown in panel C (*, P < 0.05 by ANOVA and Dunnett’s posttest).