Skip to main content
. 2009 Nov 3;151(1):103–114. doi: 10.1210/en.2009-0997

Figure 6.

Figure 6

Inhibition of AMPK increases nuclear localization of FOXO1 and impairs effects of EGCG (E) to cause nuclear translocation of FOXO1. Bar graphs represent the mean ± sem of four independent experiments. A, Cells were scored in a blinded fashion based on the appearance of cytoplasmic HA immunoreactivity (see supplemental Fig. S1). Compound C (CC) pretreatment reduced basal and EGCG-stimulated HA-Foxo1 in the cytoplasm (*, P < 0.0001 by two-way ANOVA), whereas EGCG treatment increased cytoplasmic HA immunoreactivity (†, P = 0.0009 by two-way ANOVA). B, Cells were scored based on the appearance of perinuclear HA-WT-Foxo1. EGCG treatment tended to increase perinuclear Foxo1 localization (P = 0.056 by two-way ANOVA), whereas pretreatment of cells with Compound C almost completely blocked perinuclear localization of Foxo1 and substantially inhibited the effect of EGCG to increase perinuclear localization of Foxo1 (*, P = 0.01 by two-way ANOVA). C, Cells were scored based on the appearance of nuclear HA-WT-Foxo1 localization. Compound C pretreatment significantly increased nuclear Foxo1 localization in the absence or presence of EGCG treatment, whereas EGCG treatment alone significantly reduced nuclear Foxo1 localization when compared with vehicle-treated (Veh) cells (*, P < 0.0001 and †, P = 0.0012 by two-way ANOVA for Compound C and EGCG treatments, respectively).