Figure 1.

A, Schema illustrating the design for experiment 2 of this study. Two-year-old control and prenatal T-treated ewes were ovariectomized (OVX) and over the following 2 months received an artificial hormonal regimen that simulated the normal estrous cycle and, just before killing, the midluteal phase of the estrous cycle. E₂, One 1-cm-long E₂ implant; 4 E₂, four 3-cm-long E₂ implants. See text for additional details. B, Mean LH (ng/ml) and LH pulse frequency (pulses/8 h) in control and prenatal T females taken from samples collected every 20 min during the midluteal phase of the estrous cycle before ovariectomy in control (n = 5) and prenatal T (n = 4) animals. Mean LH levels and pulse frequency were significantly higher in prenatal T animals than in controls. *, P < 0.05.