Figure 1.

Specific primers were designed to the sequence of AKAP150 and used in RT-PCR to determine expression (A). PCR on cDNA from GT1-1, GT1-7 cells, and mouse cortex show expression of AKAP150. B, Whole-cell extracts from untransfected COS 7 cells or cells transiently transfected with AKAP150-RFP cDNA alone or with the control luciferase (Luc) siRNA, AKAP150 siRNA no. 1, or AKAP150 siRNA no. 2, respectively, were immunoblotted with anti-AKAP150 antibody. Both AKAP-siRNA no. 1 and AKAP150-siRNA no. 2 but not Luc-siRNA inhibited the expression of AKAP150 in transiently transfected COS 7 cells. WB, Western blot; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. C, AKAP150-RFP transfected into GT1-1 cells is expressed at the plasma membrane.