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Quantitative RT-PCR showed that Ad-AKAP150-siRNA decreased AKAP150 mRNA expression by approximately 74%. GT1-1 cells were infected with Ad-AKAP150-siRNA (5 MOI) or Ad-Luc-siRNA (5 MOI) and RNA was isolated 48 h after infection. The data were analyzed using the comparative threshold cycle (Ct) method with β-actin as the reference gene. The data, expressed as a ratio of the control AKAP150 mRNA in uninfected cells, represent the mean ± sem from three separate experiments.
