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. 2009 Nov 3;151(1):281–290. doi: 10.1210/en.2009-0894

Figure 3.

Figure 3

Infection with Ad-AKAP150-siRNA but not Ad-Luc-siRNA inhibited the expression of AKAP150 protein in GT1-1 cells. A, Immunoblot of membrane extracts with antibodies to AKAP150 or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in GT1-1 cells. Lane 1, Uninfected GT1-1 cells; lane 2, GT1-1 cells infected with Ad-Luc-siRNA 5 MOI; lane 3, GT1-1 cells infected with Ad-AKAP150-siRNA 5 MOI. WB, Western blot. B, Immunoblot data were quantitated and represented as the percent of the uninfected GT1-1 cells (lane 1 above). Error bars, mean ± sem from different exposures of two separate experiments.