Figure 4.
Effect of Ad-AKAP150-siRNA on [Ca2+]i. Fura-2 Ca2+ imaging experiments in uninfected GT1-1 neurons (A) or GT1-1 cells infected with 5 MOI of Ad-AKAP150-siRNA (B) and Ad-Luc-siRNA (C). The tracings at the top of each section (A, B, and C) show the changes in [Ca2+]i vs. time in a representative cell. The raster plots show [Ca2+]i vs. time for 26 representative cells from a single field. The gray scale represents the [Ca2+]i from 0 to 600 nm. Forskolin (FSK; 10 μm) treatment is indicated by the bar.