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Spontaneous GnRH release in uninfected GT1-1 neurons (A), neurons infected for 48 h with 5 MOI of Ad-Luc-siRNA (B), or 5 MOI of Ad-AKAP150-siRNA (C). Samples were obtained every 2 min for 180 min from perifused cells. Data are shown for three of seven experiments for each treatment. GnRH levels were measured by RIA and data were analyzed for pulsatile secretion using cluster analysis (*, pulse). IPI, Interpulse interval.
