Figure 1.

Estrogen and lung inflammation in female mice. A, Histology of lung sections stained with hematoxylin-eosin obtained from CAR-treated sham, ovx, or ovx females mice treated with E₂ (ovx+E₂). Representative samples are shown with magnifications on the right. B, As measure of tissue morphology, a histological score was assigned to each animal through the assessment of histological parameters during tissue observation at the microscope, as described in Materials and Methods. Sham, Open boxes; ovx, black boxes; ovx+E₂, dashed boxes. C, Pleural exudate was recovered and analyzed for infiltrated PMN cell number; bar legend as in B). Bars represent the average ± sem of all the animals (n = 8), each analyzed in triplicate. *, P < 0.05; ***, P < 0.001.