Figure 1.

Expression profiles of mouse Gpr149. A, Semiquantitative multitissue RT-PCR demonstrates that Gpr149 transcripts are abundant in oocytes (Oo) but also found in brain (Br) and at lower levels in stomach (St) and small intestine (In). B, RT-PCR in ovaries of different ages shows an increase of Gpr149 during folliculogenesis. C, In the ovary, Gpr149 is found only in oocytes and transcript levels decrease after fertilization. Gapdh and Hprt1 were used as loading controls. He, Heart; Li, liver; Sp, spleen; Lu, lung; Ki, kidney; Sk, skeletal muscle; Te, testis; Ov, ovary; Nb, newborn; Ad, adult; GC, granulosa cell; GV, germinal vesicle; M-II, meiosis-II oocyte; 1-C, one-cell embryo; 2-C, two-cell embryo.