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. 2009 Nov 11;151(1):32–42. doi: 10.1210/en.2009-0855

Figure 2.

Figure 2

Tissue-selective regulation of aromatase activity by calcitriol. A, Calcitriol decreases aromatase activity in MCF-7 cells. MCF-7 cells were treated with vehicle (Cal, 0) or various concentrations of calcitriol (Cal, 0.1–100 nm) in serum-free medium containing the aromatase substrate T (100 nm) for 48 h. Aromatase activity was determined by measuring the concentrations of E2 formed in the conditioned media by an EIA and expressed as picograms of E2 formed per hour per milligram protein. **, P < 0.01 and ***, P < 0.001 compared with vehicle. B, Calcitriol decreases aromatase activity in other BCa cells. ZR-75-1 and MDA-MB 231 cells were treated with 10 or 100 nm calcitriol and aromatase activity was determined as in A. *, P < 0.05 and ***, P < 0.001 compared with vehicle. C, Calcitriol increases aromatase activity in MG-63 cells. MG-63 human osteosarcoma cells were treated with 10 and 100 nm calcitriol and aromatase activity was determined as in A. ***, P < 0.01 compared with vehicle.