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. 2009 Nov 24;151(1):134–142. doi: 10.1210/en.2009-0560

Figure 1.

Figure 1

Making of POMC neuron-specific Asb-4-expressing transgenic construct. The POMC neuronal enhancer (9–13 kb upstream of mouse POMC transcriptional unit), minimal herpes simplex virus TK promoter, and Myc epitope-tagged (13 amino acids) Asb-4 cDNA were subcloned into a pCMV-Myc vector. The POMC neuron-specific Asb-4-expressing cosset with a SV40 polyadenylation signal was cut out from the vector. Transgenic mice were made by injecting the construct DNA into the pronucleus of the fertilized eggs of C57BL mice.