Figure 2.

Effect of insulin and leptin on hypothalamic Asb-4 mRNA expression. A and B, Insulin was administered icv to rats (8 mU in 5 μl saline or 5 μl vehicle). In a second series of experiments, 8-wk-old male mice were fasted for 24 h with water ad libitum. C and D, Leptin (3 μg/g body weight) or vehicle was injected twice ip (1100 and 1500 h). Doses of insulin or leptin were used that have been demonstrated to inhibit food intake in rats or mice, respectively. Animals were killed 2 h after the second injection, and under magnification, the PVN and ARC were obtained using a brain punch. Data are expressed as mean ± sem for groups of six animals each. *, P < 0.05; **, P < 0.01 vs. control injection.