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. 2009 Nov 24;151(1):134–142. doi: 10.1210/en.2009-0560

Figure 3.

Figure 3

Expression of Myc-Asb-4 in the ARC. A, Myc-Asb-4 positivity within the ARC of transgenic animals. B, Colocalization of Myc-Asb-4 and POMC in mouse ARC cells; upper left, high-resolution image depicting Myc-Asb-4 cells (green); upper right, immunohistochemical staining for POMC (red); lower left, nuclear 4′,6-diamidino-2-phenylindole staining; lower right, merged images, illustrating colocalization (orange) of Myc-Asb-4 and POMC. Scale bar, 20 μm. C, Absence of Myc-Asb-4 in POMC-expressing cells (red) of the pituitary. D, Asb-4 mRNA levels in the ARC of wild-type and transgenic animals. **, P < 0.01.