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. 2009 Dec 17;10:140. doi: 10.1186/1471-2350-10-140

Figure 3.

Figure 3

A: Analysis of the splicing pattern by RT-PCR of the wild-type and IVS6+12_18dupTTTTGAG constructs after transfection in the HeLa cell line: both constructs reproduce the same splicing pattern (amplicon length 382 bp). Mk: 100 bp molecular ladder; WT: wild-type sample; MT: IVS6+12_18dupTTTTGAG sample. B: Analysis of the splicing pattern by RT-PCR of the wild-type and 843C>T SNP constructs after transfection in the HeLa cell line, showing that both constructs reproduce the same splicing pattern (amplicon length 432 bp). Mk: 100 bp molecular ladder; WT: wild-type sample; MT: 843C>T sample.