Table 2.
List of primers used for the generation and verifications of SPI mutants in S. Enteritidis.
| SPI deletion | Primer * | Sequence 5' - 3' |
|---|---|---|
| SPI1 | avrA_44F | TTATCGTTTAGCATAACGGCATTGTTATCGAATCGCTCATAAAGGTGTAGGCTGGAGCTGCTTC |
| SPI1 | avrA_44R | ATGATATTTTCGGTGCAGGAGCTATCATGTGGAGGGAAAAGTATCATATGAATATCCTCCTTAG |
| SPI1 | avrA_FCTR | AGACTTATATTCAGCTATCC |
| SPI1 | avrA_RCTR | ACATAACCCTGCTGTACCTG |
| SPI1 | invH_44Fw | AATATGAAAAAATTTTATAGCTGTCTTCCTGT CTTTTTACTGATCTTCGGAATAGGAACTTCAT |
| SPI1 | invH_R44 | ATGAGTTGCTCTTCATCTTCTTTCGAACGCAT GTATTGTGGATGCATATGAATATCCTCCTTAG |
| SPI1 | invH_FCTR | CAGGAGTTTTTTTTGCTAGC |
| SPI1 | invH_RCTR | CATGGGCAGCAAGTAACGTC |
| SPI2 | STM1379_44F | TCAATCGAGCAACTTTTTGCCTTCCAGGTCGATGGCGATGTTTTGTGTAGGCTGGAGCTGCTTC |
| SPI2 | STM1379_44R | ATCATGAAAAAAGTCAAAAAATTGTCTCTTACCGATTTAGTGCTCATATGAATATCCTCCTTAG |
| SPI2 | STM1379_FCTR | ACCATTCAAGAGACAATTGG |
| SPI2 | STM1379_RCTR | GTCCTGTTCTGGTACTACGC |
| SPI2 | ssaU_44Fw | ATGAGCGAGAAAACAGAACAGCCTACAGAAAAGAAATTACGTGAACTTCGGAATAGGAACTTCA |
| SPI2 | ssaU_44R | TTATGGTGTTTCGGTAGAATGCGCATAATCTA TCTTCATCACCACATATGAATATCCTCCTTAG |
| SPI2 | ssaU_FCTF | TATGGTATTAGCCGATCTGG |
| SPI2 | ssaU_RCTR | ACCTTTATCGTCAAGCACTG |
| SPI3 | STM3752_44F | TCAACGTATAGAGCCATCCGGATAAAGATACATGCCTCCCTCCAGTGTAGGCTGGAGCTGCTTC |
| SPI3 | sugR_44R | TCTTCATGCGGCGGCTGTTCTCCTCGCTTGTC GAGCATCCAGGTCATATGAATATCCTCCTTAG |
| SPI3 | STM3752_FCTR | GGATATCGTCTGCAAAGAAG |
| SPI3 | sugR_RCTR | CTAGATGTTCACGGTAGCTA |
| SPI3 | mgtC_44Fw | TTATTGACTATCAATGCTCCAGTGAATTGCGGTGATATTATCGTACTTCGGAATAGGAACTTCA |
| SPI3 | mgtC_44R | CCAGAAAAAATGGAGGAACGTATGTTAATGTTTCCTTATATTTACATATGAATATCCTCCTTAG |
| SPI3 | mgtC_FCTR | ATGAATCCCCAAAATTAAGG |
| SPI3 | mgtC_RCTR | AATCATCTGGCAAGTTAACG |
| SPI4 | STM4257_44F | ATGGAAGACGAAAGTAATCCGTGGCCTAGTTTTGTTGATACATTGTGTAGGCTGGAGCTGCTTC |
| SPI4 | STM4257_44R | TCACTCTGACACCTTTTTATTAATAGTCGTGATAATAGCTTTAC CATATGAATATCCTCCTTAG |
| SPI4 | STM4257_FCTR | TAAAGCGTATTGGTAGCAGG |
| SPI4 | STM4257_RCTR | TAATGCACACAAAGAACCTG |
| SPI4 | STM4262_44Fw | CGTATAGCCGATATTCCAATATTTATTATATT TCTCATTGTTATACTTCGGAATAGGAACTTCA |
| SPI4 | STM4262_44R | TAAACTCATCTAAGTTATCAAAAATTTGCTTCTCGGTATTCTCACATATGAATATCCTCCTTAG |
| SPI4 | STM4262_FCTR | CAGTCTATCACAGCAAGGCA |
| SPI4 | STM4262_RCTR | TTATCCGGAGAACAATCACG |
| SPI5 | SPI5_44F | ATATCGGGGAAAACAGGTGTATCTGCGGTATT TAATCTATATGTGTGTAGGCTGGAGCTGCTTC |
| SPI5 | SPI5_44R | GAAGATAAAACGATGCAAAATGCGCAGACGCT CGCCCGTCGCCTCATATGAATATCCTCCTTAG |
| SPI5 | SPI5_FCTR | ATATGCGTAACTCATCAGTC |
| SPI5 | SPI5_RCTR | GCTGCAAACGCTGGTTATGC |
* Primers "...44..." were used for amplification with pKD3 or pKD4 plasmid templates. Sequence used for annealing with the plasmids is underlined. Primers "...CTR..." were used in control PCRs, forward always with c1 or k1, and reverse always with c2 or k2.