Table 4.
List of primers used for the quantification of chicken cytokines after the infection with S. Enteritidis.
| Primer | Sequence 5' - 3' | Product size (bp) | Reference |
|---|---|---|---|
| IL-8For | ATGAACGGCAAGCTTGGAGCT | 94 | this study |
| IL-8Rev | GCAGCTCATTCCCCATCTT | ||
| TNFαFor | AATTTGCAGGCTGTTTCTGC | 112 | this study |
| TNFαRev | TATGAAGGTGGTGCAGATGG | ||
| IL-12βFor | TGGTCCACGCTTTGCAGAT | 140 | [25] |
| IL-12βRev | AAGGTTAAGGCGTGGCTTCTTA | ||
| IL-18For | ACGTGGCAGCTTTTGAAGAT | 88 | this study |
| IL-18Rev | GCGGTGGTTTTGTAACAGTG | ||
| iNOSFor | GAACAGCCAGCTCATCCGATA | 103 | [25] |
| iNOSRev | CCCAAGCTCAATGCACAACTT | ||
| IFNγFor | GCCGCACATCAAACACATATCT | 207 | [25] |
| IFNγRev | TGAGACTGGCTCCTTTTCCTT | ||
| GAPDHFor | GTCAGCAATGCATCGTGCA | 102 | [25] |
| GAPDHRev | GGCATGGACAGTGGTCATAAGA |