Table 1.
Primer Set | Primer name | 5'-3' sequence |
---|---|---|
GP5+/GP6+ | GP5+ | TTT GTT ACT GTG GTA GAT ACT AC |
GP6+ | GAA AAA TAA ACT GTA AAT CAT ATT C | |
MY09/11 | MY09 | CGT CCM ARR GGA WAC TGA TC |
MY11 | GCM CAG GGW CAT AAY AAT GG | |
PGMY09/11 | PGMY11-A | GCA CAG GGA CAT AAC AAT GG |
PGMY11-B | GCG CAG GGC CAC AAT AAT GG | |
PGMY11-C | GCA CAG GGA CAT AAT AAT GG | |
PGMY11-D | GCC CAG GGC CAC AAC AAT GG | |
PGMY11-E | GCT CAG GGT TTA AAC AAT GG | |
PGMY09-F | CGT CCC AAA GGA AAC TGA TC | |
PGMY09-G | CGA CCT AAA GGA AAC TGA TC | |
PGMY09-H | CGT CCA AAA GGA AAC TGA TC | |
PGMY09-I | G CCA AGG GGA AAC TGA TC | |
PGMY09-J | CGT CCC AAA GGA TAC TGA TC | |
PGMY09-K | CGT CCA AGG GGA TAC TGA TC | |
PGMY09-L | CGA CCT AAA GGG AAT TGA TC | |
PGMY09-M | CGA CCT AGT GGA AAT TGA TC | |
PGMY09-N | CGA CCA AGG GGA TAT TGA TC | |
PGMY09-P | G CCC AAC GGA AAC TGA TC | |
PGMY09-Q | CGA CCC AAG GGA AAC TGG TC | |
PGMY09-R | CGT CCT AAA GGA AAC TGG TC | |
HMB01 | GCG ACC CAA TGC AAA TTG GT | |
GH2O | GAA GAG CCA AGG ACA GGT AC | |
PCO4 | CAA CTT CAT CCA CGT TCA CC |
Primer sets, names and sequences used for the detection of HPV in clinical samples. GP5+/GP6+ and MY09/11 PCRs consist of a single forward and reverse primers, whereas the PGMY09/11 set comprises 5 forward (11A-E) and 13 reverse (09F-HMB01) primers and includes GH2O and PCO4 β-actin internal controls.