Table 3.

Nested PCR with the PGMY-GP system on previously negative or inconclusive results after primary PCR screen.

Sample	PGMY-GP nested PCR	HPV sequenced	Linear Array
C4	+	58	52m, 58
C5	+	87	-
V2post	-	ND	45, 54
V4on	+	16	54
V4post	+	16	16, 54
V6on	+	81	81
V6post	+	16	16

Samples negative or weakly positive for HPV using any of the three PCR primer sets were re-amplified using a PGMY-GP5+/GP6+ nested approach. Positive samples were directly sequenced to determine HPV type and the result compared to Linear Array.