Table 4.
HPV detection and typing of 15 HNSCC and 3 RRP patients.
| HPV detection method | ||||||
|---|---|---|---|---|---|---|
| Patient | Age | Diagnosis | PGMY 09/11 | Linear Array | GP5+/GP6+ nest | |
| 1 | 52 | SCC | - | - | HPV 6 | |
| 2 | 46 | SCC | - | - | HPV 6 | |
| 3 | 49 | SCC | - | - | HPV 16, mixed | |
| 4 | 58 | RRP | - | - | - | |
| 5 | 68 | SCC | - | HPV 16 | HPV 16 | |
| 6 | 31 | RRP | HPV 11 | HPV 11 | HPV 11 | |
| 7 | 61 | SCC | - | - | - | |
| 8 | 68 | SCC | - | - | - | |
| 9 | 54 | SCC | - | HPV 16 | HPV 6 | |
| 10 | 64 | SCC | - | - | HPV 6 | |
| 11 | 72 | SCC | - | - | HPV 16 | |
| 12 | 73 | SCC | - | HPV 11 | HPV 6 | |
| 13 | 87 | RRP | - | - | - | |
| 14 | 69 | SCC | - | - | - | |
| 15 | 74 | SCC | - | - | - | |
| 16 | 50 | SCC | - | - | HPV 40 | |
| 17 | 78 | SCC | - | - | mixed types | |
| 18 | 74 | SCC | - | - | - | |
| HPV +ve HNSCC detection | 0/15 | 3/15 | 10/15 | |||
PCR detection, using the PGMY09/11 primer set was unable to detect the presence of HPV in any of the head and neck squamous cell carcinoma (SCC) samples (0/15). Improved detection was achieved using the Linear Array assay (3/15) and following a nested PCR of the PGMY reaction with the GP5+/GP6+ primer set (10/15). HPV was only detected in 1/3 of recurrent respiratory papillomatosis (RRP) samples, irrespective of the method used. Samples were scored as positive if HPV was detected using one or more of the methods employed.