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. 1998 Dec 22;95(26):15270–15274. doi: 10.1073/pnas.95.26.15270

Figure 1.

Figure 1

Functional reconstitution of salt-stripped HIV-1 PICs with uninfected cell extract. Integration reactions were deproteinized and analyzed by Southern blotting. Lane 1, integration activity of untreated, gradient-purified PICs. Lane 2, HIV-1 PICs treated with 1.2 M KCl and then purified by spin column chromatography and gradient centrifugation before the integration assay. Lane 3, BSA included in the reconstitution buffer. Lanes 4–6 included 5, 10, and 15 μl, of crude cell extract, corresponding to approximately 35, 70, and 105 μg of total protein, respectively. cDNA, 9.7-kb HIV-1 linear DNA substrate; IP, 15.1-kb integration product. Approximately 3-fold more material was loaded in lanes 2–6, accounting for the increased level of cDNA.