Table 1.
Oligo name | Sequence | Purpose |
cwinv4-1 F | ACATCCACAAAACCAGCAAAC | cwinv4-1 genotyping |
cwinv4-1 R | ACAATGAACGGTTCAGCATTC | cwinv4-1 genotyping |
cwinv4-2 F | AAATGTGAACGTAAACTGCGG | cwinv4-2 genotyping |
cwinv4-2 R | TTTGTTAAATGTTTTTGGCCG | cwinv4-2 genotyping |
LBb1.3 | ATTTTGCCGATTTCGGAAC | T-DNA left border, genotyping |
AtCWINV4 RT-F | CAACGGTGTCAGATTCATTAG | AtCWINV4 RT PCR |
AtCWINV4 RT-R | CGGTACGAGTATTACACGC | AtCWINV4 RT PCR |
BrCWINV4 RT-F | TCAGGCCGATGTGGAAGTGACATT | BrCWINV4 RT PCR |
BrCWINV4 RT-R | ATCCACAAAGCCAGCAAACGATGG | BrCWINV4 RT PCR |
AtGAPDH-F | AGGGTGGTGCCAAGAAGGTTG | AtGAPDH RT PCR |
AtGAPDH-R | GTAGCCCCACTCGTTGTCGTA | AtGAPDH RT PCR |
BrUBQ-F | TTGAGGTGGAAAGCTCTGACACGA | BrUBQ RT PCR |
BrUBQ-R | AATCGGCCAATGTACGACCATCCT | BrUBQ RT PCR |
AtCWINV4-GFP-F | gaattcGAAATCAGAGTCACTGTGCC | Cloning of AtCWINV4 into pORE-R4, EcoRI site |
AtCWINV4-GFP-R | actagtTTCCTAATTTCACAGTATCTCTC | Cloning of AtCWINV4 into pORE-R4, SpeI site |