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. 2009 Oct 27;61(2):395–404. doi: 10.1093/jxb/erp309

Table 1.

Oligonucleotide primers used in this study

Oligo name Sequence Purpose
cwinv4-1 F ACATCCACAAAACCAGCAAAC cwinv4-1 genotyping
cwinv4-1 R ACAATGAACGGTTCAGCATTC cwinv4-1 genotyping
cwinv4-2 F AAATGTGAACGTAAACTGCGG cwinv4-2 genotyping
cwinv4-2 R TTTGTTAAATGTTTTTGGCCG cwinv4-2 genotyping
LBb1.3 ATTTTGCCGATTTCGGAAC T-DNA left border, genotyping
AtCWINV4 RT-F CAACGGTGTCAGATTCATTAG AtCWINV4 RT PCR
AtCWINV4 RT-R CGGTACGAGTATTACACGC AtCWINV4 RT PCR
BrCWINV4 RT-F TCAGGCCGATGTGGAAGTGACATT BrCWINV4 RT PCR
BrCWINV4 RT-R ATCCACAAAGCCAGCAAACGATGG BrCWINV4 RT PCR
AtGAPDH-F AGGGTGGTGCCAAGAAGGTTG AtGAPDH RT PCR
AtGAPDH-R GTAGCCCCACTCGTTGTCGTA AtGAPDH RT PCR
BrUBQ-F TTGAGGTGGAAAGCTCTGACACGA BrUBQ RT PCR
BrUBQ-R AATCGGCCAATGTACGACCATCCT BrUBQ RT PCR
AtCWINV4-GFP-F gaattcGAAATCAGAGTCACTGTGCC Cloning of AtCWINV4 into pORE-R4, EcoRI site
AtCWINV4-GFP-R actagtTTCCTAATTTCACAGTATCTCTC Cloning of AtCWINV4 into pORE-R4, SpeI site