Fig. 7.

Time-course changes of the expression of the 23 TB unigenes in the roots during Cd treatment. TB plants were hydroponically grown and CdCl₂ was added to the culture solutions at a final concentration of 0.1 μM. The roots were harvested 3 h (Cd3h), 1 d (Cd1d), or 3 d (Cd3d) after the start of treatment, together with untreated roots (Cd0). The relative amount of transcripts of individual TB unigenes in the roots was determined by real-time RT-PCR. Expression levels of the unigenes in Cd-treated roots relative to those in untreated roots were calculated using a unigene TVR18P14C (a ubiquitin gene) as a standard control. Bars indicate standard errors (n=4).