. 2009 Oct 16;61(2):423–437. doi: 10.1093/jxb/erp313

Table 1.

Sequences of polynucleotides used in construction of expression tag libraries

Name of polynucleotide	Nucleotide sequence
Biotinylated adaptor oligo(dT) primer	Biotin-5′-CTGATCTAGAGGTACCGGATCCCAGCAGT₁₇-3′
Adaptor 1_GT for Cd0 library	5′-ACAGGTTCAGAGTTCTACAGTCCGACATGGTCAGCAGCATG-3′
	3′-CAAGTCTCAAGATGTCAGGCTGTACCAGTCGTC-P-5′
Adaptor 1_CT for Cd3h library	5′-ACAGGTTCAGAGTTCTACAGTCCGACATGCTCAGCAGCATG-3′
	3′-CAAGTCTCAAGATGTCAGGCTGTACAGGTCGTC-P-5′
Adaptor 1_AC for Cd1d library	5′-ACAGGTTCAGAGTTCTACAGTCCGACATGACCAGCAGCATG-3′
	3′-CAAGTCTCAAGATGTCAGGCTGTACTGGTCGTC-P 5′
Adaptor 1_TC for Cd3d library	5′-ACAGGTTCAGAGTTCTACAGTCCGACATGTCCAGCAGCATG-3′
	3′-CAAGTCTCAAGATGTCAGGCTGTACAGGTCGTC-P-5′
Adaptor 2	5′-CAAGCAGAAGACGGCATACGA-3′
	3′-GTTCGTCTTCTGCCGTATGCTNN-P-5′
GEX PCR primer set	5′-CAAGCAGAAGACGGCATACGA-3′
	5′-AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA-3′
GEX sequencing primer	5′-CCGACAGGTTCAGAGTTCTACAGTCCGACATG-3′

Bold and underlined characters indicate library coding nucleotides and EcoP15I recognition sites, respectively. ‘-P’ indicates phosphorylated 5’ termini.