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. 2009 Nov 20;61(2):473–482. doi: 10.1093/jxb/erp320

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© 2009 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 4. — Evidence suggesting chloroplast and ROS involvement in AL-PCD. Percentage of AL-PCD, necrosis, and living cells in cultures, scored using the cell morphology marker of AL-PCD, 24 h after heat treatment. Values are means ±SD (n ≥3 replicates). (A) Dark-grown cultures and light-grown cultures, untreated and treated with the chloroplast inhibitors norflurazon (NF), spectinomycin (SM), and lincomycin (LM). PCD values marked with an asterisk were significantly different from PCD in untreated light-grown cultures (P=0.026 for dark-grown cultures and P <0.001 for NF, SM, and LM-treated cultures). (B) Light-grown cultures, control (C; untreated) and treated (T) with the antioxidants ascorbate (ASC), sodium ascorbate (Na-ASC), and reduced glutathione (GSH). PCD values marked with an asterisk were significantly different from PCD in the untreated control for that treatment (P=0.001 for ASC and Na-ASC and P <0.001 for GSH).