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. 2010 Feb;51(2):247–261. doi: 10.1194/jlr.R000687

Fig. 6.

Fig. 6.

The bisretinoid A2-DHP-PE exhibits fluorescence intensity that is greater than that of A2E when considered relative to the absorbance peaks of each compound. Reverse-phase HPLC chromatogram using C18 column. Overlay of UV-visible absorbance (black) and fluorescence (red; excitation, 430 nm, emission, 600 nm) profiles. AU, absorbance units; a.u., arbitrary units of fluorescence intensity. Insets above, absorbance spectra of A2E, isoA2E, and A2-DHP-PE. B: Chromatogram in A is expanded between retention times 40–50 min; Inset above, absorbance spectra of all-trans-retinal dimer-PE (atRALdi-PE).