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. 2010 Feb;51(2):309–317. doi: 10.1194/jlr.M000729

Fig. 3.

Fig. 3.

Desnutrin C-terminal deletion mutants lack TAG hydrolase activity in cells. Immunoblots of full-length GFP-tagged desnutrin (DES-GFP) or GFP-tagged truncated desnutrin (W367×-GFP and Q289×-GFP) in COS-7 cells (A) or 3T3-L1CARΔ (C). TAG hydrolysis measured by 14C-TAG content remaining in COS-7 cells (B) or 3T3-L1CARΔ adipocytes (D) pulse labeled with [U-14C]palmitate, washed, and then infected with GFP, desnutrin-GFP, or GFP-tagged desnutrin C-terminal deletion mutants prior to chase with unlabeled medium for 60 h. Values are means ± SEM from two to three separate experiments. *P < 0.05,**P < 0.01, and ***P < 0.001.