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. 2010 Feb;51(2):334–344. doi: 10.1194/jlr.M000513

Fig. 1.

Fig. 1.

Surfactant lipids downregulate the production of LPS-induced IL-8 in A549 cells. A: ELISA results for IL-8 released by A549 cells pretreated with Survanta®, Curosurf®, or DPPC for 2 h at 0 to 500 μg/ml and stimulated with LPS (100 ng/ml) in the presence of sCD14 (250 ng/ml) for 18 h. Pretreatment of cells with Survanta® (≥50μg/ml), Curosurf® (≥50 μg/ml), or DPPC (≥100 μg/ml) significantly suppressed the release of IL-8. * P < 0.05 versus no surfactant. B: IL-8 released by A549 cells pretreated with Survanta® (250 μg/ml), Curosurf® (250 μg/ml), or DPPC (500 μg/ml) for 2 h and then stimulated with LPS (100 ng/ml) in the presence of sCD14 (250 ng/ml) for 18 h. Induction of IL-8 was significantly reduced when the cells were preincubated with the surfactant preparations. C and D: RT-PCR for IL-8 mRNA expression in A549 cells pretreated with Survanta® or Curosurf® (250 μg/ml) (C) or liposomal DPPC (500μg/ml) (D) for 2 h followed by stimulation with LPS for 4 h in the presence of sCD14. The graphs show the densitometric analyses of the IL-8 mRNA relative to GAPDH mRNA control. Values are mean ± SD (n = 4). *P < 0.05 versus cells without surfactant pretreatment.