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. 2010 Feb;51(2):388–399. doi: 10.1194/jlr.M001461

Fig. 7.

Fig. 7.

Localization of ECFP-PLCδ1-PH and EYFP-cPLA2α during phagocytosis in human macrophages. Human macrophages, cotransfected with the ECFP-PLCδ1-PH and the EYFP-cPLA2α constructs, were subjected to synchronized phagocytosis using Alexa Fluor 594-labeled opsonized zymosan, fixed at 0 (Control) and 15 min, and analyzed by confocal microscopy (A). B: A cell with high expression of the construct ECFP-PLCδ1-PH is shown. In A and B, middle panels, fluorescence intensities are shown in pseudocolor, and detailed fluorescence in forming phagosomes is shown in panels to the right.