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Analysis of human macrophages transfected with EGFP-cPLA2α during phagocytosis of zymosan. Human macrophages transfected with the construction EGFP-cPLA2α were treated with opsonized zymosan for 20 min, fixed, and fluorescence analyzed by confocal microscopy. A: Ten different z-stacks of the same cell are shown (from top to bottom). B: Pseudocolor analysis of the intensity of fluorescence of the cell in A is shown. C and D: Detailed phagosomes from B.
