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Analysis of a human macrophage transfected with EGFP-PLCδ1-PH during phagocytosis of zymosan. Human macrophages transfected with the construction EGFP- PLCδ1-PH were treated with zymosan for 20 min, fixed, and fluorescence analyzed by confocal microscopy. A: Ten different z-stacks of the same cell are shown (from top to bottom). B: Pseudocolor analysis of the intensity of fluorescence from some phagosomes is shown (white square in A).
