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After 4 weeks of a diet supplemented with 0.1% cholesterol, 1% methionine and 5% peanut oil, rabbit abdominal aorta showed severe endothelial dysfunction. Endothelium-dependent relaxation in response to acetylcholine significantly decreased by 72% at 0.1 μM acetylcholine (P < 0.05), by 60% at 0.3 μM acetylcholine (P < 0.001) and by 63% at 1 μM acetylcholine (P < 0.001) compared to control arteries. Mean ± SEM. n = 4 per group. *P < 0.05 vs. control and †P < 0.001 vs. control.
