Fig. 1.

Characterization of adherent cells isolated from UCB and UC. a, c Flow cytometric analysis of cell surface markers of MSC from UCB and UC, respectively. Representative histograms are demonstrated, and their respective controls are shown by the black lines. b, d Morphology of adherent cells isolated from UCB and UC. After 15 days in culture, all isolated MSC populations displayed a spindle-shaped morphology. e Differentiation potential of adherent cells isolated from UCB and UC. Osteogenic differentiation of adherent cells from UCB and UC was demonstrated by calcium deposition shown by von Kossa stain; Adipogenesis was detected by the formation of intracytoplasmic lipid droplets stained with oil red O; Cell spheres from UCB and UC were stained with toluidine blue to confirm chondrogenic differentiation. Mucopolysaccharide-rich extracellular matrix is shown in pinkish metachromatic areas. Abbreviations: CD cluster of differentiation; HLA-ABC human leukocyte antigen-ABC; HLA-DR human leukocyte antigen-DR; UCB umbilical cord blood; UC umbilical cord