Figure 1.

Chaperonin-assisted reactivation of heat-denatured mtMDH. mtMDH (0.3 μM) was heat- and DTT-denatured in the absence (⊞), or in the presence of GroEL (4 μM). The time-dependent reactivation of the MDH was measured at 25°C without additional cofactors (▵) or with supplemented ATP (1 mM; ○), GroES (12 μM) and ADP (1 mM) (◊), or GroES (12 μM) and ATP (1 mM; □). (Inset) Threefold enlargement of the slow kinetics.