Fig. 2.

Progenitor-product relationship among MDP, CDP and pre-cDC. (A) Phenotype of myeloid progenitor (MP, Lin⁻ Sca-1⁻ c-Kit^high CX₃CR1⁻), MDP (CX₃CR1⁺ c-Kit^hi) and CDP (CD115⁺CX₃CR1⁺c-Kit^lo) in Cx₃cr1^gfp/+ mice. (B) Donor-derived bone marrow cells analyzed by flow cytometry for presence of MDPs, CDPs and pre-cDCs two days after intra-bone marrow transfer of 5×10⁴ MPs, MDPs or CDPs. (C) Donor-derived splenocytes analyzed for the presence of cDCs, pDCs and monocytes 7 days after transfer of MDPs or CDPs. (D) EGFP expression in indicated cell populations in LysM-Cre x Rosa26-StopfloxEGFP reporter mice (fig. S8). EGFP index = % of EGFP⁺ cells / % of EGFP⁺ Gr1^high blood monocytes (20). In (C) and (D) left side shows representative dot plots. Bar graphs summarize 2-4 independent experiments with 3-4 mice. Error bars represent the mean ± SEM.