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HCN4 stably expressed in HEK293 cells. (Panel A) Detecting HCN4 protein from a HCN4 stable cell line (right) but not from HEK293 cell protein (left). (Panel B) Confocal image of HCN4 stable cell lines immunostained with anti-HCN4 antibody. (Panel C) Representative current traces recorded from HCN4 stable cell lines before (black) and after (red) perfusion of 2 mM Cs+.
