Table 2.
Clinical chemical analyses for Ad-PDGF-B delivery *
| Clinical Chemical Parameters |
Prior to surgery | Day3 | Day 7 | Day 14 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Col | L-Ad | H-Ad | Col | L-Ad | H-Ad | Col | L-Ad | H-Ad | Col | L-Ad | H-Ad | |
| Albumin (g/dl) | 2.900 (0.200) | 3.100 (0.210) | 2.750 (0.055) | 2.733 (0.216) | 2.783 (0.223) | 2.917 (0.223) | 2.667 (0.216) | 2.600 (0.126) | 2.917 (0.117) | 2.750 (0.243) | 2.700 (0.420) | 2.783 (0.194) |
| ALP (U/l) | 200.67 (29.49) | 253.50 (28.81) | 207.50 (36.30) | 183.67 (45.70) | 195.50 (45.05) | 141.17 (30.64) | 177.83 (41.80) | 163.50 (28.39) | 192.17 (40.63) | 204.00 (46.43) | 227.83 (51.34) | 200.00 (36.78) |
| ALT (U/l) | 89.67 (7.74) | 88.17 (6.68) | 90.33 (8.55) | 75.00 (8.60) | 76.67 (13.31) | 69.50 (3.78) | 87.50 (22.82) | 85.50 (7.23) | 89.83 (15.96) | 85.83 (10.46) | 78.83 (8.11) | 89.67 (11.27) |
| Amylase (U/l) | 2182.17 (119.59) | 2054.5 (333.84) | 2019.67 (209.93) | 1706.67 (256.08) | 1335.00 (246.33) | 1487.50 (155.96) | 1779.00 (189.74) | 1589.50 (232.52) | 1764.17 (188.13) | 1893.17 (226.83) | 1742.00 (504.32) | 1945.67 (219.46) |
| AST (U/l) | 81.33 (16.67) | 78.33 (9.42) | 80.83 (12.95) | 91.50 (12.42) | 115.00 (42.68) | 88.33 (17.10) | 97.83 (23.70) | 71.50 (10.88) | 98.00 (11.51) | 73.00 (9.38) | 85.50 (10.58) | 83.50 (14.15) |
| Bilirubin (mg/dl) | 19.67 (1.37) | 21.83 (1.47) | 23.33 (1.86) | 23.33 (2.25) | 24.00 (1.67) | 20.67 (1.03) | 21.67 (1.63) | 19.33 (1.03) | 22.83 (1.47) | 21.67 (1.86) | 29.67 (14.60) | 22.33 (2.07) |
| Calcium (mg/dl) | 11.18 (0.70) | 10.78 (0.23) | 10.63 (0.15) | 10.28 (0.16) | 10.32 (0.23) | 10.60 (0.23) | 10.47 (0.22) | 10.57 (0.29) | 10.35 (0.25) | 10.52 (0.22) | 10.58 (0.26) | 10.80 (0.27) |
| Cholesterol (mg/dl) | 87.17 (17.97) | 87.50 (13.07) | 81.00 (7.69) | 99.33 (14.31) | 106.83 (13.12) | 95.33 (7.31) | 83.00 (20.95) | 83.83 (9.81) | 82.33 (7.45) | 90.50 (17.07) | 93.17 (16.10) | 84.33 (15.34) |
| Creatine Kinase (U/l) | 105.83 (11.86) | 94.67 (12.04) | 104.50 (36.54) | 426.5 (72.45) | 403.50 (146.06) | 153.00 (119.30) | 302.50 (132.63) | 115.67 (55.85) | 346.33 (117.08) | 83.50 (35.80) | 244.83 (110.49) | 94.83 (22.48) |
| Creatinine (mg/dl) | 0.283 (0.041) | 0.383 (0.041) | 0.383 (0.041) | 0.383 (0.041) | 0.400 (0.063) | 0.333 (0.082) | 0.433 (0.234) | 0.367 (0.052) | 0.400 (0) | 0.350 (0.055) | 1.700 (3.184) | 0.400 (0) |
| Glucose (mg/dl) | 181.00 (18.98) | 187.33 (3.44) | 283.33 (52.30) | 225.17 (48.06) | 251.00 (77.69) | 226.33 (39.62) | 243.17 (127.82) | 275.83 (33.58) | 209.83 (23.20) | 255.83 (58.81) | 223.83 (62.07) | 295.83 (40.92) |
| Phosphorus (mg/dl) | 5.983 (0.313) | 5.767 (0.497) | 5.300 (0.498) | 5.567 (0.383) | 5.533 (0.524) | 5.700 (0.704) | 5.733 (0.625) | 5.250 (0.367) | 5.983 (0.417) | 5.700 (0.228) | 6.500 (1.942) | 5.467 (0.372) |
| T. Bilirubin (mg/dl) | 0.167 (0.052) | 0.117 (0.041) | 0.167 (0.082) | 0.117 (0.041) | 0.217 (0.240) | 0.400 (0.642) | 0.250 (0.207) | 0.167 (0.103) | 0.183 (0.075) | 0.200 (0.155) | 0.150 (0.122) | 0.217 (0.117) |
| Total Protein (g/dl) | 6.517 (0.256) | 6.550 (0.217) | 6.017 (0.117) | 6.217 (0.343) | 6.267 (0.344) | 6.467 (0.484) | 6.233 (0.207) | 5.850 (0.207) | 6.300 (0.141) | 6.183 (0.204) | 6.150 (0.689) | 6.067 (0.314) |
| Globulin (g/dl) | 3.617 (0.407) | 3.483 (0.041) | 3.283 (0.147) | 3.467 (0.186) | 3.483 (0.133) | 3.567 (0.273) | 3.533 (0.695) | 3.250 (0.152) | 3.400 (0.632) | 3.383 (0.117) | 3.560 (0.114) | 3.300 (0.141) |
*
All comparisons to collagen group.
n=6/group. The number in this table demonstrates the average value of parameters from the each group and the number in the parentheses refers to the standard deviations. Neither significant differences nor value out of normal range were noted among the Ad-PDGF-B and collagen matrix groups during early time points, as well as beyond 14 days (data not shown). Abbreviations used: Col:collagen matrix only group, L-Ad: 5.5×108 PFU/ml Ad-PDGF-B treated group, H-Ad: 5.5×109 PFU/ml Ad-PDGF-B treated group; ALP: alkaline phosphatase; ALT: alanine transaminase; AST: aspartate transaminase; T. Bilirubin: total bilirubin