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. Author manuscript; available in PMC: 2010 Jul 7.
Published in final edited form as: Oncogene. 2009 Oct 5;29(1):45–55. doi: 10.1038/onc.2009.307

Figure 5. Acquisition of invasive properties of II-4 cells upon RalA or Exo84 knockdown is associated with a decrease in E-cadherin protein expression.

Figure 5

(A) Scrambled (a), RalA knockdown (b), RalB knockdown (c), RalA/RalB double-knockdown (d), Exo84 knockdown (e), Sec5 knockdown (f), or RalBP-1 knockdown (g) HaCaT-II-4 cells were used to populate tissues and frozen sections were subjected to immunofluorescent analysis with anti-E-cadherin antibody (red). DAPI was used to counterstain the nuclei. Invading cells in representative images are shown with arrows. (B) Western blot analysis of homogenized epithelium from tissues shown in (A). Total Erk is shown as a loading control. E-cadherin expression protein (C) and mRNA (D) were measured in the linear range by densitometry or real-time PCR, respectively. * p < 0.05 for sh-RalA, shRalA/B, and sh-Exo84 compared to sh-Scram using Mann-Whitney test.