Table 5.
Summary of the direction of fluorescence changes for cation-induced folding of Tel22.
ODN | Location | Stackeda | Mobility | Change in fluorescence intensity (U → F) |
Change in polarization (U → F) |
Change in fluorescence intensity for kinetic steps |
---|---|---|---|---|---|---|
Na+ (50 mM) | ||||||
AP1 | core | yes | fixed | b↓ | ↑ | c↓↓↓ |
AP7 | loop 1 | no | mobile | ↑ | ↑ | ↑↑ |
AP13 | loop 2 | yes | fixed | ↓ | ↑ | ↓↓ |
AP19 | loop 3 | yes | mobile | ↑ | ↓ | ↓↑ |
K+ (3 mM) | ||||||
AP1 | core | ↓ | ↑ | ↑↓→ | ||
AP7 | loop 1 | ↑ | → | ↑↓↓ | ||
AP13 | loop 2 | ↓ | → | ↑↓↓ | ||
AP19 | loop 3 | ↑ | ↓ | ↑↓↑ | ||
K+ (33 mM) | ||||||
AP1 | core | → | ↑ | ↑↓ | ||
AP7 | loop 1 | ↑ | → | ↑↓ | ||
AP13 | loop 2 | ↓ | → | ↓ | ||
AP19 | loop 3 | ↑ | ↓ | ↑↓ |
Determined from the NMR structure of Tel22 (14)
↑ = increase, ↓ = decrease, → = no significant change.
In order of steps 1, 2 and 3.