Transections of sunflower cotyledons at early developmental stages: (A) ‘Prosol 35’ at 4–5 daa—cells show meristematic features throughout the tissue; (B) ‘Prosol 35’ at 6–7 daa—oil bodies become visible; (C) and (D) ‘Dakar’ and ‘11051’, respectively—at 8–9 daa oil bodies occupy only the cell periphery; (E) and (F) ‘Prosol 35’ at 10–12 daa and 14–15 daa, respectively. Fixation with 3 % glutaraldehyde, included in JB4 resin and stained with Sudan black 0·1 %. n, Nucleus; o, oil body; p, protein body. Scale bars = 10 μm.