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Deficient activation of INO1, GAL1, and PHO5 transcripts in rpb5–9. RNA was prepared from cells grown at 30°C under nonactivating (−) or activating (+) conditions. RNA also was prepared from cells grown at 37°C for GAL1 activation. ACT1 (actin mRNA) and U3 (RNA polymerase II transcribed snRNA) were included as loading controls.
