Figure 6.

RPB5 and the CTD have overlapping functions. (A) Equal amounts of yeast cells were spotted onto YPD plates and tested for growth at the temperatures indicated. The RPB5/CTD double mutant failed to grow at 37°C (indicated by the arrow). (B) Growth curves of isogenic wt and mutant strains grown at the permissive (30°C) and nonpermissive temperatures (37°C). (C) RNA prepared from cells grown at, or shifted to, the indicated temperatures. U3, snRNA transcript of RNA polymerase II used as loading control. (D) Quantification and normalization of the results in C.