Table 2.
Oligonucleotide Pairs for the Interrogation of TEV Protease Recognition Sequence P1' Position (ENLYFQX)
| X = | Forward primer sequence (5' - 3') | Reverse primer sequence (5' - 3') |
|---|---|---|
| A | CTAGCGAGAACCTGTACTTCCAGGCCG | GATCCGGCCTGGAAGTACAGGTTCTCG |
| C | CTAGCGAGAACCTGTACTTCCAGTGCG | GATCCGCACTGGAAGTACAGGTTCTCG |
| D | CTAGCGAGAACCTGTACTTCCAGGACG | GATCCGTCCTGGAAGTACAGGTTCTCG |
| E | CTAGCGAGAACCTGTACTTCCAGGAGG | GATCCCTCCTGGAAGTACAGGTTCTCG |
| F | CTAGCGAGAACCTGTACTTCCAGTTCG | GATCCGAACTGGAAGTACAGGTTCTCG |
| G | CTAGCGAGAACCTGTACTTCCAGGGCG | GATCCGCCCTGGAAGTACAGGTTCTCG |
| H | CTAGCGAGAACCTGTACTTCCAGCACG | GATCCGTGCTGGAAGTACAGGTTCTCG |
| I | CTAGCGAGAACCTGTACTTCCAGATCG | GATCCGATCTGGAAGTACAGGTTCTCG |
| K | CTAGCGAGAACCTGTACTTCCAGAAGG | GATCCCTTCTGGAAGTACAGGTTCTCG |
| L | CTAGCGAGAACCTGTACTTCCAGCTGG | GATCCCAGCTGGAAGTACAGGTTCTCG |
| M | CTAGCGAGAACCTGTACTTCCAGATGG | GATCCCATCTGGAAGTACAGGTTCTCG |
| N | CTAGCGAGAACCTGTACTTCCAGAACG | GATCCGTTCTGGAAGTACAGGTTCTCG |
| P | CTAGCGAGAACCTGTACTTCCAGCCAG | GATCCTGGCTGGAAGTACAGGTTCTCG |
| Q | CTAGCGAGAACCTGTACTTCCAGCAGG | GATCCCTGCTGGAAGTACAGGTTCTCG |
| R | CTAGCGAGAACCTGTACTTCCAGCGCG | GATCCGCGCTGGAAGTACAGGTTCTCG |
| S | CTAGCGAGAACCTGTACTTCCAGAGCG | GATCCGCTCTGGAAGTACAGGTTCTCG |
| T | CTAGCGAGAACCTGTACTTCCAGACCG | GATCCGGTCTGGAAGTACAGGTTCTCG |
| V | CTAGCGAGAACCTGTACTTCCAGGTGG | GATCCCACCTGGAAGTACAGGTTCTCG |
| W | CTAGCGAGAACCTGTACTTCCAGTGGG | GATCCCCACTGGAAGTACAGGTTCTCG |
| Y | CTAGCGAGAACCTGTACTTCCAGTACG | GATCCGTACTGGAAGTACAGGTTCTCG |