Table 1.
Proteins of Pseudopolydora vexillosa larvae showing differential expressions when attaining competence.
| No. | Acc. No. | Proteins identified | Mascot score (No. peptides matched) | kDa | pI | Fold Change | Unique peptides |
|---|---|---|---|---|---|---|---|
| 1 | gi|47550939 | Calreticulin | 78(6) | 49 | 4.2 | 1.9 | GLQTSEDAR VHVIFNYK KVHVIFNYK VHVIFNYKGK EEFNDGDGWKSR IMFPPDICGPGTK |
| 2 | gi|32967463 | 90-kDa heat-shock protein precursor | 105(4) | 66 | 5.1 | 1.56 | DYVTRMK VIKDILDK TKPIWMR ELISNSSDALDKIR |
| 3 | gi|4468655 | Cytoplasmic intermediate filament protein | 86(10) | 69 | 5.7 | 3.86 | ELAALAYR LLEGEENR FLEAQNRK DMQDLNER LQDSLNKAR LLDDAEKEK KLLEGEENR VRFLEAQNR KDMQDLNER AMYQAELDEAR |
| 4 | gi|46909257 | ATP synthase beta subunit | 303(8) | 37 | 5.1 | 1.62 | IQWFLSQ IGLFGGAGVGK VVDLLAPYAK GGKIGLFGGAGVGK AHGGYSVFAGVGER VSLVYGQMNEPPGAR GIAELGIYPAVDPLDSNSR IPSAVGYQPTLATDMGTMQER |
| 5 | gi|47117349 | Tropomyosin | 149(8) | 33 | 4.6 | 1.59 | ATEAERQVVK LAITEVDLER KLAITEVDLER LEEASKAADESER EVDRLEDELLAEK QITQLESDVGALQRR ENAQDLAEQMEQKLK LTTTENNFDVANEQLQEANVK |
| 6 | gi|59894747 | Actin | 66(8) | 42 | 5.4 | 1.60 | CDVDIRK IIAPPERK AGFAGDDAPR GYSFTTTAER AVFPSIVGRPR DSYVGDEAQSKR QEYDESGPSIVHR SYELPDGQVITIGNER |
| 7 | gi|61651838 | Tyrosine 3-monooxygenase activation protein | 120(8) | 29 | 4.8 | 2.89 | NLLSVAYK IISSIEQK DEDLSVEER TAPQDAEEEK YDEMVESMK DSTLIMQLLR AASDIAMTELPPTHPIR LAEQAERYDEMVESMK |
| 8 | gi|178045 | Gamma-actin | 442(13) | 26 | 5.7 | 1.53 | CDVDIRK IIAPPERK DLTDYLMK IKIIAPPER GYSFTTTAER KITALAPSTMK QEYDESGPSIVHR DLTDYLMKILTER GYSFTTTAEREIVR LDLAGRDLTDYLMK QEYDESGPSIVHRK SYELPDGQVITIGNER DLYANTVLSGGTTMYPGIADR |
| 9 | gi|11596085 | Troponin C | 50(2) | 17 | 4.3 | 1.5 | ELKEIFR MSLNCKADWLEK |
| 10 | gi|6981214 | Matrix metallo-peptidase 7 | 54(8) | 30 | 7.7 | -3.61 | FFGLPETGK KFYLHDSK FYLHDSKTK VMEIMQKPR MAAMRLTLFR MWSMQIPLNFK MWSMQIPLNFKR ALRMWSMQIPLNFK |
| 11 | gi|467525 | T cell receptor delta pre-chain | 53(8) | 31 | 9.5 | 5.53 | EPKVYAEK NGTNVACLVK EIGSAIVISPSK YSINFQKEAK VNMMSLTVLGLR YLCALSEPNIR VNMMSLTVLGLRK SGAINFLLTAKLFFS |
The proteins listed above expressed abundantly across 3 developmental stages but had at least a 1.5-fold change in spot volume at the competent larval stage. Mascot scores > 50 are considered significant.